Yap energy storage

Does energy starvation induce Yap phosphorylation?

Energy-stress-induced YAP phosphorylation was also observed in myoblast C2C12, mammary epithelial MCF10A, and the cervical cancer HeLa cell lines (Supplementary Fig. 1d–f). Figure 1: Cellular energy starvation activates Lats and inhibits YAP. (a) Glucose starvation induces YAP phosphorylation.

Does cellular energy stress affect Yap and TAZ activity?

Collectively our data suggest that cellular energy stress inhibits YAP and TAZ activity. The increase of YAP Ser 127 phosphorylation indicates that Lats kinase activity is enhanced by energy stress (Fig. 1a). Phosphorylation of Ser 909 and Thr 1079 in Lats1 positively correlates with Lats activity 33, 34.

Does AMPK-induced Yap inhibition suppress oncogenic transformation of lats-null cells?

AMPK-induced YAP inhibition can suppress oncogenic transformation of Lats-null cells with high YAP activity. Our study establishes a molecular mechanism and functional significance of AMPK in linking cellular energy status to the Hippo–YAP pathway.

Does AMPK phosphorylate endogenous Yap?

Moreover, the AMPK activators AICAR and metformin increased the association of endogenous YAP and AMPK (Fig. 3f). Although the precise molecular basis for the energy-stress-induced interaction between AMPK and YAP is unknown, our data suggest a direct role of AMPK in YAP phosphorylation.

Is Yap a tumour suppressor?

For instance, YAP could function as a tumour suppressor in some cell types, such as haematological cancers, by inducing apoptosis in response to DNA damage 17, 18, 19. Recent studies have revealed a large number of extra- and intracellular signals that modulate YAP/TAZ activity.

How does cAMP/PKA signalling affect Yap?

Kim, M. et al. cAMP/PKA signalling reinforces the LATS–YAP pathway to fully suppress YAP in response to actin cytoskeletal changes.